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标题: 头穴透刺对脑出血大鼠血肿周围脑组织神经生长因子表达的影响 [打印本页]

作者: 刘李静 时间: 2018-5-31 16:21
标题: 头穴透刺对脑出血大鼠血肿周围脑组织神经生长因子表达的影响
[size=10.000000pt]目的 [size=10.000000pt]观察头穴透刺法对脑出血大鼠血肿周围脑组织神经生长因子[size=10.000000pt]( [size=10.000000pt]NGF[size=10.000000pt]) [size=10.000000pt]表达的影响[size=10.000000pt]。[size=10.000000pt]方法[size=10.000000pt]健康雄性 [size=10.000000pt]Wistar [size=10.000000pt]大鼠[size=10.000000pt]120 [size=10.000000pt]只[size=10.000000pt]，[size=10.000000pt]按照随机数字表法分为假手术组[size=10.000000pt]、[size=10.000000pt]模型组[size=10.000000pt]、[size=10.000000pt]针刺组[size=10.000000pt]，[size=10.000000pt]每组 [size=10.000000pt]40 [size=10.000000pt]只[size=10.000000pt]，[size=10.000000pt]每组再随机分为[size=10.000000pt]6 h[size=10.000000pt]、[size=10.000000pt]24 h[size=10.000000pt]、[size=10.000000pt]3 d[size=10.000000pt]、[size=10.000000pt]7 d 4 [size=10.000000pt]个时点[size=10.000000pt]。[size=10.000000pt]采用改良的自体动脉血法制备大鼠脑出血模型[size=10.000000pt]，[size=10.000000pt]给予[size=10.000000pt]“[size=10.000000pt]百会[size=10.000000pt]”[size=10.000000pt]透[size=10.000000pt]“[size=10.000000pt]太阳[size=10.000000pt]”[size=10.000000pt]穴电针干预[size=10.000000pt]。[size=10.000000pt]通过 [size=10.000000pt]Longa [size=10.000000pt]评分法进行神经行为学评估[size=10.000000pt]，[size=10.000000pt]免疫组织化学法检测血肿周围脑组织 [size=10.000000pt]NGF [size=10.000000pt]的阳性细胞表达[size=10.000000pt]，[size=10.000000pt]q-PCR [size=10.000000pt]法检测 [size=10.000000pt]NGF mRNA [size=10.000000pt]表达量[size=10.000000pt]。[size=10.000000pt]结果 [size=10.000000pt]与假手术组比较[size=10.000000pt]，[size=10.000000pt]模型组各时点神经行为学评分升高[size=10.000000pt]，[size=10.000000pt]NGF [size=10.000000pt]阳性细胞数增多[size=10.000000pt]，[size=10.000000pt]NGF mRNA [size=10.000000pt]表达上调[size=10.000000pt]，[size=10.000000pt]差异有统计学意义[size=10.000000pt]([size=10.000000pt]P < 0. 05[size=10.000000pt]) ; 与模型组比较，针刺组 [size=10.000000pt]6 h [size=10.000000pt]神经行为学评分[size=10.000000pt]、[size=10.000000pt]NGF [size=10.000000pt]的阳性细胞数[size=10.000000pt]，[size=10.000000pt]NGF mRNA [size=10.000000pt]表达均无明显变化[size=10.000000pt]，[size=10.000000pt]差异无统计学意义[size=10.000000pt]([size=10.000000pt]P > 0. 05[size=10.000000pt]) ，[size=10.000000pt]针刺组 [size=10.000000pt]24 h[size=10.000000pt]、[size=10.000000pt]3 d[size=10.000000pt]、[size=10.000000pt]7 d [size=10.000000pt]神经[size=10.000000pt]行为学评分降低[size=10.000000pt]，[size=10.000000pt]NGF [size=10.000000pt]阳性细胞数增多[size=10.000000pt]，[size=10.000000pt]NGF mRNA [size=10.000000pt]表达上调[size=10.000000pt]，[size=10.000000pt]差异有统计学意义 [size=10.000000pt]([size=10.000000pt]P < 0. 05[size=10.000000pt]) [size=10.000000pt]。[size=10.000000pt]结论 [size=10.000000pt]“[size=10.000000pt]百会[size=10.000000pt]”[size=10.000000pt]透[size=10.000000pt]“[size=10.000000pt]太阳[size=10.000000pt]”[size=10.000000pt]头穴透刺通过上调 [size=10.000000pt]NGF [size=10.000000pt]基因及蛋白的表达[size=10.000000pt]，[size=10.000000pt]促进脑出血大鼠神经功能恢复[size=10.000000pt]，[size=10.000000pt]具有良好的神经保护作用[size=10.000000pt]。

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